She was born Anna Maria Gallo, the daughter of Francesco Gallo and Barbara Basinsin, in the Quartieri Spagnoli (Spanish Quarter) of Naples, a red-light district of the city, still known for its high crime. According to tradition, another saint, the Jesuit, Francis de Geronimo, predicted her future sanctity while she was still an infant. Her family was of the middle class, but her father, a weaver of gold lace, was a very violent man, who regularly abused his family physically, often severely. When Gallo was sixteen, her father attempted to force her into a marriage with a young man of means who was seeking her hand. She refused and asked to join the Franciscan Third Order, through which she could live out a religious life in the family home. The friars of Naples were part of the reform of St. Peter of Alcantara, and they and the tertiares under their rule were known for the strictness of their lives. Through the intervention of a friar, Father Theophilus, permission to enter the Order was eventually granted by her father. Gallo was received into the Order on 8 September 1731 and began wearing the religious habit of the Order, which was an uncommon practice by that era. She also adopted the use of the religious name she took upon being received into the Order, out of her devotion to the Blessed Mother, St. Francis of Assisi and the Passion of Christ. She continued to live in the family home to serve God as a consecrated virgin, as was customary in those days. She took as her spiritual director, the Franciscan friar, John Joseph of the Cross, while her confessor was the Barnabite priest, Francis Xavier Bianchi, and she began to be known among her neighbors for her work of charity, helping the poor of the sector. She was a person of deep prayer, often spending long hours in meditation. In 1753 she joined with another Franciscan tertiary, known only as Maria Felice, and they moved into a small palace owned by a priest, Giovanni Pessiri, who became their spiritual director. The two women occupied the second floor, sleeping on the floor, and the priest the floor above. She is said to have received the wounds of the stigmata while living there and suffered patiently many physical afflictions and spiritual trials.She would wear gloves to cover the marks on her hands, while she did her work. She is also said to have had apparitions of Saint Raphael the Archangel, who healed her of several afflictions.